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Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotianatabacum

Photosystem II from transplastomic plants of Nicotiana tabacum with a hexahistidine tag at the N-terminal end of the PsbE subunit (α-chain of the cytochrome b(559)) was purified according to the protocol of Fey et al. (BBA 12:1501–1509, 2008). The protein sample was then subjected to two additional...

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Detalles Bibliográficos
Autores principales: Piano, Dario, El Alaoui, Sabah, Korza, Henryk J., Filipek, Renata, Sabala, Izabela, Haniewicz, Patrycja, Buechel, Claudia, De Sanctis, Daniele, Bochtler, Matthias
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005121/
https://www.ncbi.nlm.nih.gov/pubmed/21063907
http://dx.doi.org/10.1007/s11120-010-9597-x