A Two-Hybrid Assay to Study Protein Interactions within the Secretory Pathway

Interactions of transcriptional activators are difficult to study using transcription-based two-hybrid assays due to potent activation resulting in false positives. Here we report the development of the Golgi two-hybrid (G2H), a method that interrogates protein interactions within the Golgi, where t...

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Detalles Bibliográficos
Autores principales: Dube, Danielle H., Li, Bin, Greenblatt, Ethan J., Nimer, Sadeieh, Raymond, Amanda K., Kohler, Jennifer J.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011011/
https://www.ncbi.nlm.nih.gov/pubmed/21209940
http://dx.doi.org/10.1371/journal.pone.0015648

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011011/
https://www.ncbi.nlm.nih.gov/pubmed/21209940
http://dx.doi.org/10.1371/journal.pone.0015648

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