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A Two-Hybrid Assay to Study Protein Interactions within the Secretory Pathway

Interactions of transcriptional activators are difficult to study using transcription-based two-hybrid assays due to potent activation resulting in false positives. Here we report the development of the Golgi two-hybrid (G2H), a method that interrogates protein interactions within the Golgi, where t...

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Detalles Bibliográficos
Autores principales:	Dube, Danielle H., Li, Bin, Greenblatt, Ethan J., Nimer, Sadeieh, Raymond, Amanda K., Kohler, Jennifer J.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011011/ https://www.ncbi.nlm.nih.gov/pubmed/21209940 http://dx.doi.org/10.1371/journal.pone.0015648

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