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Detection of Predation Using qPCR: Effect of Prey Quantity, Elapsed Time, Chaser Diet, and Sample Preservation on Detectable Quantity of Prey DNA

Using quantitative PCR that amplified a prey-specific mtDNA 214 bp amplicon from the COI mitochondrial gene of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), prey eggs of known age and number were fed to larvae of the generalist predator lady beetle Coleomeg...

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Detalles Bibliográficos
Autores principales: Weber, Donald C., Lundgren, Jonathan G.
Formato: Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011838/
https://www.ncbi.nlm.nih.gov/pubmed/19619033
http://dx.doi.org/10.1673/031.009.4101