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Immunopanning purification and long-term culture of human retinal ganglion cells

PURPOSE: To establish a robust method to isolate primary retinal ganglion cells (RGCs) from human fetal retina for long-term culture while maintaining neuronal morphology and marker protein expression. METHODS: A total of six human retinas were obtained from aborted fetuses at 10 to 12 weeks of gest...

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Detalles Bibliográficos
Autores principales: Zhang, Xin-Mei, Li Liu, David Ta, Chiang, Sylvia Wai-Yee, Choy, Kwong-Wai, Pang, Chi-Pui, Lam, Dennis Shun-Chiu, Yam, Gary Hin-Fai
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012647/
https://www.ncbi.nlm.nih.gov/pubmed/21203402