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Amp-PCR: Combining a Random Unbiased Phi29-Amplification with a Specific Real-Time PCR, Performed in One Tube to Increase PCR Sensitivity

In clinical situations where a diagnostic real-time PCR assay is not sensitive enough, leading to low or falsely negative results, or where detection earlier in a disease progression would benefit the patient, an unbiased pre-amplification prior to the real-time PCR could be beneficial. In Amp-PCR,...

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Detalles Bibliográficos
Autores principales: Erlandsson, Lena, Nielsen, Lars Peter, Fomsgaard, Anders
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013126/
https://www.ncbi.nlm.nih.gov/pubmed/21209824
http://dx.doi.org/10.1371/journal.pone.0015719