Comparative Production Analysis of Three Phlebovirus Nucleoproteins under Denaturing or Non-Denaturing Conditions for Crystallographic Studies

Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies. To overcome this problem, we set up a standard protocol in which production...

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Detalles Bibliográficos
Autores principales: Lantez, Violaine, Dalle, Karen, Charrel, Rémi, Baronti, Cécile, Canard, Bruno, Coutard, Bruno
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014985/
https://www.ncbi.nlm.nih.gov/pubmed/21245924
http://dx.doi.org/10.1371/journal.pntd.0000936

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014985/
https://www.ncbi.nlm.nih.gov/pubmed/21245924
http://dx.doi.org/10.1371/journal.pntd.0000936

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