Use of RNAlater in fluorescence-activated cell sorting (FACS) reduces the fluorescence from GFP but not from DsRed

BACKGROUND: Flow cytometry utilizes signals from fluorescent markers to separate targeted cell populations for gene expression studies. However, the stress of the FACS process could change normal gene expression profiles. RNAlater could be used to stop such changes in original gene expression profil...

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Detalles Bibliográficos
Autores principales: Zaitoun, Ismail, Erickson, Christopher S, Schell, Kathy, Epstein, Miles L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017069/
https://www.ncbi.nlm.nih.gov/pubmed/21134271
http://dx.doi.org/10.1186/1756-0500-3-328

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017069/
https://www.ncbi.nlm.nih.gov/pubmed/21134271
http://dx.doi.org/10.1186/1756-0500-3-328

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