DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

BACKGROUND: Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment. To overcome this problem, an RNA of interest can be fused into a stable bacterial RNA for the resulting chimeric construct to accumulate...

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Detalles Bibliográficos
Autores principales: Liu, Yamei, Stepanov, Victor G, Strych, Ulrich, Willson, Richard C, Jackson, George W, Fox, George E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019158/
https://www.ncbi.nlm.nih.gov/pubmed/21134283
http://dx.doi.org/10.1186/1472-6750-10-85

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019158/
https://www.ncbi.nlm.nih.gov/pubmed/21134283
http://dx.doi.org/10.1186/1472-6750-10-85

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