Removal of PCR Error Products and Unincorporated Primers by Metal-Chelate Affinity Chromatography

Immobilized Metal Affinity Chromatography (IMAC) has been used for decades to purify proteins on the basis of amino acid content, especially surface-exposed histidines and “histidine tags” genetically added to recombinant proteins. We and others have extended the use of IMAC to purification of nucle...

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Detalles Bibliográficos
Autores principales: Kanakaraj, Indhu, Jewell, David L., Murphy, Jason C., Fox, George E., Willson, Richard C.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021510/
https://www.ncbi.nlm.nih.gov/pubmed/21264292
http://dx.doi.org/10.1371/journal.pone.0014512

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021510/
https://www.ncbi.nlm.nih.gov/pubmed/21264292
http://dx.doi.org/10.1371/journal.pone.0014512

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