Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

BACKGROUND: Cell biologists face the need to rapidly analyse their proteins of interest in order to gain insight into their function. Often protein purification, cellular localisation and Western blot analyses can be multi-step processes, where protein is lost, activity is destroyed or effective ant...

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Detalles Bibliográficos
Autores principales: Kim, Ji Hun, Chang, Tsz M, Graham, Alison N, Choo, K HA, Kalitsis, Paul, Hudson, Damien F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022668/
https://www.ncbi.nlm.nih.gov/pubmed/21194474
http://dx.doi.org/10.1186/1471-2091-11-50

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022668/
https://www.ncbi.nlm.nih.gov/pubmed/21194474
http://dx.doi.org/10.1186/1471-2091-11-50

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