Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

BACKGROUND: Disulfide bonds are one of the most common post-translational modifications found in proteins. The production of proteins that contain native disulfide bonds is challenging, especially on a large scale. Either the protein needs to be targeted to the endoplasmic reticulum in eukaryotes or...

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Detalles Bibliográficos
Autores principales: Nguyen, Van Dat, Hatahet, Feras, Salo, Kirsi EH, Enlund, Eveliina, Zhang, Chi, Ruddock, Lloyd W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022669/
https://www.ncbi.nlm.nih.gov/pubmed/21211066
http://dx.doi.org/10.1186/1475-2859-10-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022669/
https://www.ncbi.nlm.nih.gov/pubmed/21211066
http://dx.doi.org/10.1186/1475-2859-10-1

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