Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system

The porcine parvovirus (PPV) VP2 protein was expressed in an insect-baculovirus cell system and was purified using Ni-NTA affinity column chromatography. The recombinant 6-His-tagged VP2 protein with molecular mass (Mr) of about 64 kDa was detected by anti-his antibody and anti-PPV serum. Electron m...

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Detalles Bibliográficos
Autores principales: Zhou, Hongchao, Yao, Guizhe, Cui, Shangjin
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022681/
https://www.ncbi.nlm.nih.gov/pubmed/21143963
http://dx.doi.org/10.1186/1743-422X-7-366

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022681/
https://www.ncbi.nlm.nih.gov/pubmed/21143963
http://dx.doi.org/10.1186/1743-422X-7-366

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