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Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system
The porcine parvovirus (PPV) VP2 protein was expressed in an insect-baculovirus cell system and was purified using Ni-NTA affinity column chromatography. The recombinant 6-His-tagged VP2 protein with molecular mass (Mr) of about 64 kDa was detected by anti-his antibody and anti-PPV serum. Electron m...
Autores principales: | Zhou, Hongchao, Yao, Guizhe, Cui, Shangjin |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022681/ https://www.ncbi.nlm.nih.gov/pubmed/21143963 http://dx.doi.org/10.1186/1743-422X-7-366 |
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