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Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells

BACKGROUND: Extracellular matrix receptors of the integrin family are known to regulate cell adhesion, shape and functions. The α8 integrin chain is expressed in glomerular mesangial cells and in vascular smooth muscle cells. Mice deficient for α8 integrin have structural alterations in glomeruli bu...

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Autores principales: Marek, Ines, Volkert, Gudrun, Jahn, Angelika, Fahlbusch, Fabian, Zürn, Christina, Özcan, Zehra, Goppelt-Struebe, Margarete, Hilgers, Karl F, Rascher, Wolfgang, Hartner, Andrea
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022721/
https://www.ncbi.nlm.nih.gov/pubmed/21194485
http://dx.doi.org/10.1186/1471-2121-11-102
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author Marek, Ines
Volkert, Gudrun
Jahn, Angelika
Fahlbusch, Fabian
Zürn, Christina
Özcan, Zehra
Goppelt-Struebe, Margarete
Hilgers, Karl F
Rascher, Wolfgang
Hartner, Andrea
author_facet Marek, Ines
Volkert, Gudrun
Jahn, Angelika
Fahlbusch, Fabian
Zürn, Christina
Özcan, Zehra
Goppelt-Struebe, Margarete
Hilgers, Karl F
Rascher, Wolfgang
Hartner, Andrea
author_sort Marek, Ines
collection PubMed
description BACKGROUND: Extracellular matrix receptors of the integrin family are known to regulate cell adhesion, shape and functions. The α8 integrin chain is expressed in glomerular mesangial cells and in vascular smooth muscle cells. Mice deficient for α8 integrin have structural alterations in glomeruli but not in renal arteries. For this reason we hypothesized that mesangial cells and vascular smooth muscle cells differ in their respective capacity to compensate for the lack of α8 integrin. RESULTS: Wild type and α8 integrin-deficient mesangial cells varied markedly in cell morphology and expression or localization of cytoskeletal molecules. In α8 integrin-deficient mesangial cells α-smooth muscle actin and CTGF were downregulated. In contrast, there were no comparable differences between α8 integrin-deficient and wild type vascular smooth muscle cells. Expression patterns of integrins were altered in α8 integrin-deficient mesangial cells compared to wild type mesangial cells, displaying a prominent overexpression of α2 and α6 integrins, while expression patterns of the these integrins were not different between wild type and α8 integrin-deficient vascular smooth muscle cells, respectively. Cell proliferation was augmented in α8 integrin-deficient mesangial cells, but not in vascular smooth muscle cells, compared to wild type cells. CONCLUSIONS: Our findings suggest that α8 integrin deficiency has differential effects in mesangial cells and vascular smooth muscle cells. While the phenotype of vascular smooth muscle cells lacking α8 integrin is not altered, mesangial cells lacking α8 integrin differ considerably from wild type mesangial cells which might be a consequence of compensatory changes in the expression patterns of other integrins. This could result in glomerular changes in α8 integrin-deficient mice, while the vasculature is not affected in these mice.
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spelling pubmed-30227212011-01-19 Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells Marek, Ines Volkert, Gudrun Jahn, Angelika Fahlbusch, Fabian Zürn, Christina Özcan, Zehra Goppelt-Struebe, Margarete Hilgers, Karl F Rascher, Wolfgang Hartner, Andrea BMC Cell Biol Research Article BACKGROUND: Extracellular matrix receptors of the integrin family are known to regulate cell adhesion, shape and functions. The α8 integrin chain is expressed in glomerular mesangial cells and in vascular smooth muscle cells. Mice deficient for α8 integrin have structural alterations in glomeruli but not in renal arteries. For this reason we hypothesized that mesangial cells and vascular smooth muscle cells differ in their respective capacity to compensate for the lack of α8 integrin. RESULTS: Wild type and α8 integrin-deficient mesangial cells varied markedly in cell morphology and expression or localization of cytoskeletal molecules. In α8 integrin-deficient mesangial cells α-smooth muscle actin and CTGF were downregulated. In contrast, there were no comparable differences between α8 integrin-deficient and wild type vascular smooth muscle cells. Expression patterns of integrins were altered in α8 integrin-deficient mesangial cells compared to wild type mesangial cells, displaying a prominent overexpression of α2 and α6 integrins, while expression patterns of the these integrins were not different between wild type and α8 integrin-deficient vascular smooth muscle cells, respectively. Cell proliferation was augmented in α8 integrin-deficient mesangial cells, but not in vascular smooth muscle cells, compared to wild type cells. CONCLUSIONS: Our findings suggest that α8 integrin deficiency has differential effects in mesangial cells and vascular smooth muscle cells. While the phenotype of vascular smooth muscle cells lacking α8 integrin is not altered, mesangial cells lacking α8 integrin differ considerably from wild type mesangial cells which might be a consequence of compensatory changes in the expression patterns of other integrins. This could result in glomerular changes in α8 integrin-deficient mice, while the vasculature is not affected in these mice. BioMed Central 2010-12-31 /pmc/articles/PMC3022721/ /pubmed/21194485 http://dx.doi.org/10.1186/1471-2121-11-102 Text en Copyright ©2010 Marek et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marek, Ines
Volkert, Gudrun
Jahn, Angelika
Fahlbusch, Fabian
Zürn, Christina
Özcan, Zehra
Goppelt-Struebe, Margarete
Hilgers, Karl F
Rascher, Wolfgang
Hartner, Andrea
Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
title Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
title_full Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
title_fullStr Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
title_full_unstemmed Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
title_short Lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
title_sort lack of α8 integrin leads to morphological changes in renal mesangial cells, but not in vascular smooth muscle cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022721/
https://www.ncbi.nlm.nih.gov/pubmed/21194485
http://dx.doi.org/10.1186/1471-2121-11-102
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