The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana
BACKGROUND: Throughout their lives plants produce new organs from groups of pluripotent cells called meristems, located at the tips of the shoot and the root. The size of the shoot meristem is tightly controlled by a feedback loop, which involves the homeodomain transcription factor WUSCHEL (WUS) an...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023791/ https://www.ncbi.nlm.nih.gov/pubmed/21176196 http://dx.doi.org/10.1186/1471-2229-10-285 |
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author | Wahl, Vanessa Brand, Luise H Guo, Ya-Long Schmid, Markus |
author_facet | Wahl, Vanessa Brand, Luise H Guo, Ya-Long Schmid, Markus |
author_sort | Wahl, Vanessa |
collection | PubMed |
description | BACKGROUND: Throughout their lives plants produce new organs from groups of pluripotent cells called meristems, located at the tips of the shoot and the root. The size of the shoot meristem is tightly controlled by a feedback loop, which involves the homeodomain transcription factor WUSCHEL (WUS) and the CLAVATA (CLV) proteins. This regulatory circuit is further fine-tuned by morphogenic signals such as hormones and sugars. RESULTS: Here we show that a family of four plant-specific proteins, encoded by the FANTASTIC FOUR (FAF) genes, has the potential to regulate shoot meristem size in Arabidopsis thaliana. FAF2 and FAF4 are expressed in the centre of the shoot meristem, overlapping with the site of WUS expression. Consistent with a regulatory interaction between the FAF gene family and WUS, our experiments indicate that the FAFs can repress WUS, which ultimately leads to an arrest of meristem activity in FAF overexpressing lines. The finding that meristematic expression of FAF2 and FAF4 is under negative control by CLV3 further supports the hypothesis that the FAFs are modulators of the genetic circuit that regulates the meristem. CONCLUSION: This study reports the initial characterization of the Arabidopsis thaliana FAF gene family. Our data indicate that the FAF genes form a plant specific gene family, the members of which have the potential to regulate the size of the shoot meristem by modulating the CLV3-WUS feedback loop. |
format | Text |
id | pubmed-3023791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30237912011-01-20 The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana Wahl, Vanessa Brand, Luise H Guo, Ya-Long Schmid, Markus BMC Plant Biol Research Article BACKGROUND: Throughout their lives plants produce new organs from groups of pluripotent cells called meristems, located at the tips of the shoot and the root. The size of the shoot meristem is tightly controlled by a feedback loop, which involves the homeodomain transcription factor WUSCHEL (WUS) and the CLAVATA (CLV) proteins. This regulatory circuit is further fine-tuned by morphogenic signals such as hormones and sugars. RESULTS: Here we show that a family of four plant-specific proteins, encoded by the FANTASTIC FOUR (FAF) genes, has the potential to regulate shoot meristem size in Arabidopsis thaliana. FAF2 and FAF4 are expressed in the centre of the shoot meristem, overlapping with the site of WUS expression. Consistent with a regulatory interaction between the FAF gene family and WUS, our experiments indicate that the FAFs can repress WUS, which ultimately leads to an arrest of meristem activity in FAF overexpressing lines. The finding that meristematic expression of FAF2 and FAF4 is under negative control by CLV3 further supports the hypothesis that the FAFs are modulators of the genetic circuit that regulates the meristem. CONCLUSION: This study reports the initial characterization of the Arabidopsis thaliana FAF gene family. Our data indicate that the FAF genes form a plant specific gene family, the members of which have the potential to regulate the size of the shoot meristem by modulating the CLV3-WUS feedback loop. BioMed Central 2010-12-22 /pmc/articles/PMC3023791/ /pubmed/21176196 http://dx.doi.org/10.1186/1471-2229-10-285 Text en Copyright ©2010 Wahl et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wahl, Vanessa Brand, Luise H Guo, Ya-Long Schmid, Markus The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana |
title | The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana |
title_full | The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana |
title_fullStr | The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana |
title_full_unstemmed | The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana |
title_short | The FANTASTIC FOUR proteins influence shoot meristem size in Arabidopsis thaliana |
title_sort | fantastic four proteins influence shoot meristem size in arabidopsis thaliana |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023791/ https://www.ncbi.nlm.nih.gov/pubmed/21176196 http://dx.doi.org/10.1186/1471-2229-10-285 |
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