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Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
BACKGROUND: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables that exerts anti-oxidant, anti-inflammatory, anti-cancer and radio-sensitizing activities. Nonetheless, the mechanism responsible for SFN-induced cell death is not fully understood. In the present study, anti-cancer...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024296/ https://www.ncbi.nlm.nih.gov/pubmed/21159204 http://dx.doi.org/10.1186/1477-5956-8-67 |
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author | Lee, Chan-Hee Jeong, Soo-Jin Yun, Sun-Mi Kim, Ji-Hyun Lee, Hyo-Jung Ahn, Kwang Seok Won, Suk-Hyun Kim, Hyun Seok Lee, Hyo-Jeong Ahn, Kyoo-Seok Zhu, Shudong Chen, Chang-Yan Kim, Sung-Hoon |
author_facet | Lee, Chan-Hee Jeong, Soo-Jin Yun, Sun-Mi Kim, Ji-Hyun Lee, Hyo-Jung Ahn, Kwang Seok Won, Suk-Hyun Kim, Hyun Seok Lee, Hyo-Jeong Ahn, Kyoo-Seok Zhu, Shudong Chen, Chang-Yan Kim, Sung-Hoon |
author_sort | Lee, Chan-Hee |
collection | PubMed |
description | BACKGROUND: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables that exerts anti-oxidant, anti-inflammatory, anti-cancer and radio-sensitizing activities. Nonetheless, the mechanism responsible for SFN-induced cell death is not fully understood. In the present study, anti-cancer mechanism of SFN was elucidated in LNCaP prostate cancer cells. RESULTS: SFN exerted cytotoxicity and increased TUNEL positive cells in a concentration-dependent manner in LNCaP cells. Proteomics study revealed that levels of nine proteins including tubulin β-2, phosphoglucomutase-3 (PGM3), melanoma-derived leucine zipper containing extra-nuclear factor, activin A type I receptor precursor, smoothelin-A, KIA0073, hypothetical protein LOC57691 and two unnamed proteins were changed over 8 folds in SFN treated LNCaP cells compared to untreated control. We have further confirmed that SFN reduced PGM3 expression with western blotting and showed that PGM3 siRNA enhanced cytotoxicity demonstrated by cell morphology and TUNEL assays in LNCaP cells. CONCLUSION: Taken together, these findings suggest that PGM3 plays a role in mediating SFN-induced cell death in LNCaP cells, and is a potential molecular therapeutic target for prostate cancer. |
format | Text |
id | pubmed-3024296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30242962011-01-21 Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells Lee, Chan-Hee Jeong, Soo-Jin Yun, Sun-Mi Kim, Ji-Hyun Lee, Hyo-Jung Ahn, Kwang Seok Won, Suk-Hyun Kim, Hyun Seok Lee, Hyo-Jeong Ahn, Kyoo-Seok Zhu, Shudong Chen, Chang-Yan Kim, Sung-Hoon Proteome Sci Research BACKGROUND: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables that exerts anti-oxidant, anti-inflammatory, anti-cancer and radio-sensitizing activities. Nonetheless, the mechanism responsible for SFN-induced cell death is not fully understood. In the present study, anti-cancer mechanism of SFN was elucidated in LNCaP prostate cancer cells. RESULTS: SFN exerted cytotoxicity and increased TUNEL positive cells in a concentration-dependent manner in LNCaP cells. Proteomics study revealed that levels of nine proteins including tubulin β-2, phosphoglucomutase-3 (PGM3), melanoma-derived leucine zipper containing extra-nuclear factor, activin A type I receptor precursor, smoothelin-A, KIA0073, hypothetical protein LOC57691 and two unnamed proteins were changed over 8 folds in SFN treated LNCaP cells compared to untreated control. We have further confirmed that SFN reduced PGM3 expression with western blotting and showed that PGM3 siRNA enhanced cytotoxicity demonstrated by cell morphology and TUNEL assays in LNCaP cells. CONCLUSION: Taken together, these findings suggest that PGM3 plays a role in mediating SFN-induced cell death in LNCaP cells, and is a potential molecular therapeutic target for prostate cancer. BioMed Central 2010-12-16 /pmc/articles/PMC3024296/ /pubmed/21159204 http://dx.doi.org/10.1186/1477-5956-8-67 Text en Copyright ©2010 Lee et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Lee, Chan-Hee Jeong, Soo-Jin Yun, Sun-Mi Kim, Ji-Hyun Lee, Hyo-Jung Ahn, Kwang Seok Won, Suk-Hyun Kim, Hyun Seok Lee, Hyo-Jeong Ahn, Kyoo-Seok Zhu, Shudong Chen, Chang-Yan Kim, Sung-Hoon Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells |
title | Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells |
title_full | Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells |
title_fullStr | Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells |
title_full_unstemmed | Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells |
title_short | Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells |
title_sort | down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in lncap prostate cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024296/ https://www.ncbi.nlm.nih.gov/pubmed/21159204 http://dx.doi.org/10.1186/1477-5956-8-67 |
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