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Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells

BACKGROUND: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables that exerts anti-oxidant, anti-inflammatory, anti-cancer and radio-sensitizing activities. Nonetheless, the mechanism responsible for SFN-induced cell death is not fully understood. In the present study, anti-cancer...

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Autores principales: Lee, Chan-Hee, Jeong, Soo-Jin, Yun, Sun-Mi, Kim, Ji-Hyun, Lee, Hyo-Jung, Ahn, Kwang Seok, Won, Suk-Hyun, Kim, Hyun Seok, Lee, Hyo-Jeong, Ahn, Kyoo-Seok, Zhu, Shudong, Chen, Chang-Yan, Kim, Sung-Hoon
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024296/
https://www.ncbi.nlm.nih.gov/pubmed/21159204
http://dx.doi.org/10.1186/1477-5956-8-67
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author Lee, Chan-Hee
Jeong, Soo-Jin
Yun, Sun-Mi
Kim, Ji-Hyun
Lee, Hyo-Jung
Ahn, Kwang Seok
Won, Suk-Hyun
Kim, Hyun Seok
Lee, Hyo-Jeong
Ahn, Kyoo-Seok
Zhu, Shudong
Chen, Chang-Yan
Kim, Sung-Hoon
author_facet Lee, Chan-Hee
Jeong, Soo-Jin
Yun, Sun-Mi
Kim, Ji-Hyun
Lee, Hyo-Jung
Ahn, Kwang Seok
Won, Suk-Hyun
Kim, Hyun Seok
Lee, Hyo-Jeong
Ahn, Kyoo-Seok
Zhu, Shudong
Chen, Chang-Yan
Kim, Sung-Hoon
author_sort Lee, Chan-Hee
collection PubMed
description BACKGROUND: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables that exerts anti-oxidant, anti-inflammatory, anti-cancer and radio-sensitizing activities. Nonetheless, the mechanism responsible for SFN-induced cell death is not fully understood. In the present study, anti-cancer mechanism of SFN was elucidated in LNCaP prostate cancer cells. RESULTS: SFN exerted cytotoxicity and increased TUNEL positive cells in a concentration-dependent manner in LNCaP cells. Proteomics study revealed that levels of nine proteins including tubulin β-2, phosphoglucomutase-3 (PGM3), melanoma-derived leucine zipper containing extra-nuclear factor, activin A type I receptor precursor, smoothelin-A, KIA0073, hypothetical protein LOC57691 and two unnamed proteins were changed over 8 folds in SFN treated LNCaP cells compared to untreated control. We have further confirmed that SFN reduced PGM3 expression with western blotting and showed that PGM3 siRNA enhanced cytotoxicity demonstrated by cell morphology and TUNEL assays in LNCaP cells. CONCLUSION: Taken together, these findings suggest that PGM3 plays a role in mediating SFN-induced cell death in LNCaP cells, and is a potential molecular therapeutic target for prostate cancer.
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spelling pubmed-30242962011-01-21 Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells Lee, Chan-Hee Jeong, Soo-Jin Yun, Sun-Mi Kim, Ji-Hyun Lee, Hyo-Jung Ahn, Kwang Seok Won, Suk-Hyun Kim, Hyun Seok Lee, Hyo-Jeong Ahn, Kyoo-Seok Zhu, Shudong Chen, Chang-Yan Kim, Sung-Hoon Proteome Sci Research BACKGROUND: Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables that exerts anti-oxidant, anti-inflammatory, anti-cancer and radio-sensitizing activities. Nonetheless, the mechanism responsible for SFN-induced cell death is not fully understood. In the present study, anti-cancer mechanism of SFN was elucidated in LNCaP prostate cancer cells. RESULTS: SFN exerted cytotoxicity and increased TUNEL positive cells in a concentration-dependent manner in LNCaP cells. Proteomics study revealed that levels of nine proteins including tubulin β-2, phosphoglucomutase-3 (PGM3), melanoma-derived leucine zipper containing extra-nuclear factor, activin A type I receptor precursor, smoothelin-A, KIA0073, hypothetical protein LOC57691 and two unnamed proteins were changed over 8 folds in SFN treated LNCaP cells compared to untreated control. We have further confirmed that SFN reduced PGM3 expression with western blotting and showed that PGM3 siRNA enhanced cytotoxicity demonstrated by cell morphology and TUNEL assays in LNCaP cells. CONCLUSION: Taken together, these findings suggest that PGM3 plays a role in mediating SFN-induced cell death in LNCaP cells, and is a potential molecular therapeutic target for prostate cancer. BioMed Central 2010-12-16 /pmc/articles/PMC3024296/ /pubmed/21159204 http://dx.doi.org/10.1186/1477-5956-8-67 Text en Copyright ©2010 Lee et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lee, Chan-Hee
Jeong, Soo-Jin
Yun, Sun-Mi
Kim, Ji-Hyun
Lee, Hyo-Jung
Ahn, Kwang Seok
Won, Suk-Hyun
Kim, Hyun Seok
Lee, Hyo-Jeong
Ahn, Kyoo-Seok
Zhu, Shudong
Chen, Chang-Yan
Kim, Sung-Hoon
Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
title Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
title_full Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
title_fullStr Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
title_full_unstemmed Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
title_short Down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in LNCaP prostate cancer cells
title_sort down-regulation of phosphoglucomutase 3 mediates sulforaphane-induced cell death in lncap prostate cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024296/
https://www.ncbi.nlm.nih.gov/pubmed/21159204
http://dx.doi.org/10.1186/1477-5956-8-67
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