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A Simple, Highly Efficient Method for Heterologous Expression in Mammalian Primary Neurons Using Cationic Lipid-mediated mRNA Transfection

Expression of heterologous proteins in adult mammalian neurons is a valuable technique for the study of neuronal function. The post-mitotic nature of mature neurons prevents effective DNA transfection using simple, cationic lipid-based methods. Adequate heterologous protein expression is often only...

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Detalles Bibliográficos
Autores principales: Williams, Damian J., Puhl, Henry L., Ikeda, Stephen R.
Formato: Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024561/
https://www.ncbi.nlm.nih.gov/pubmed/21267423
http://dx.doi.org/10.3389/fnins.2010.00181