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A Simple, Highly Efficient Method for Heterologous Expression in Mammalian Primary Neurons Using Cationic Lipid-mediated mRNA Transfection
Expression of heterologous proteins in adult mammalian neurons is a valuable technique for the study of neuronal function. The post-mitotic nature of mature neurons prevents effective DNA transfection using simple, cationic lipid-based methods. Adequate heterologous protein expression is often only...
Autores principales: | Williams, Damian J., Puhl, Henry L., Ikeda, Stephen R. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024561/ https://www.ncbi.nlm.nih.gov/pubmed/21267423 http://dx.doi.org/10.3389/fnins.2010.00181 |
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