DNA intercalation without flipping in the specific ThaI–DNA complex

The PD-(D/E)XK type II restriction endonuclease ThaI cuts the target sequence CG/CG with blunt ends. Here, we report the 1.3 Å resolution structure of the enzyme in complex with substrate DNA and a sodium or calcium ion taking the place of a catalytic magnesium ion. The structure identifies Glu54, A...

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Detalles Bibliográficos
Autores principales: Firczuk, Malgorzata, Wojciechowski, Marek, Czapinska, Honorata, Bochtler, Matthias
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025569/
https://www.ncbi.nlm.nih.gov/pubmed/20861000
http://dx.doi.org/10.1093/nar/gkq834

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025569/
https://www.ncbi.nlm.nih.gov/pubmed/20861000
http://dx.doi.org/10.1093/nar/gkq834

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