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The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer
BACKGROUND: Qualitative and quantitative changes in human mitochondrial DNA (mtDNA) have been implicated in various cancer types. A 4,977 bp deletion in the major arch of the mitochondrial genome is one of the most common mutations associated with a variety of human diseases and aging. METHODS: We c...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025938/ https://www.ncbi.nlm.nih.gov/pubmed/21232124 http://dx.doi.org/10.1186/1471-2350-12-8 |
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author | Chen, Tao He, Jing Shen, Lijun Fang, Hezhi Nie, Hezhongrong Jin, Tao Wei, Xiaosong Xin, Yijuan Jiang, Yulin Li, Hongzhi Chen, Guorong Lu, Jianxin Bai, Yidong |
author_facet | Chen, Tao He, Jing Shen, Lijun Fang, Hezhi Nie, Hezhongrong Jin, Tao Wei, Xiaosong Xin, Yijuan Jiang, Yulin Li, Hongzhi Chen, Guorong Lu, Jianxin Bai, Yidong |
author_sort | Chen, Tao |
collection | PubMed |
description | BACKGROUND: Qualitative and quantitative changes in human mitochondrial DNA (mtDNA) have been implicated in various cancer types. A 4,977 bp deletion in the major arch of the mitochondrial genome is one of the most common mutations associated with a variety of human diseases and aging. METHODS: We conducted a comprehensive study on clinical features and mtDNA of 104 colorectal cancer patients in the Wenzhou area of China. In particular, using a quantitative real time PCR method, we analyzed the 4,977 bp deletion and mtDNA content in tumor tissues and paired non-tumor areas from these patients. RESULTS: We found that the 4,977 bp deletion was more likely to be present in patients of younger age (≤65 years, p = 0.027). In patients with the 4,977 bp deletion, the deletion level decreased as the cancer stage advanced (p = 0.031). Moreover, mtDNA copy number in tumor tissues of patients with this deletion increased, both compared with that in adjacent non-tumor tissues and with in tumors of patients without the deletion. Such mtDNA content increase correlated with the levels of the 4,977 bp deletion and with cancer stage (p < 0.001). CONCLUSIONS: Our study indicates that the mtDNA 4,977 bp deletion may play a role in the early stage of colorectal cancer, and it is also implicated in alteration of mtDNA content in cancer cells. |
format | Text |
id | pubmed-3025938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30259382011-01-25 The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer Chen, Tao He, Jing Shen, Lijun Fang, Hezhi Nie, Hezhongrong Jin, Tao Wei, Xiaosong Xin, Yijuan Jiang, Yulin Li, Hongzhi Chen, Guorong Lu, Jianxin Bai, Yidong BMC Med Genet Research Article BACKGROUND: Qualitative and quantitative changes in human mitochondrial DNA (mtDNA) have been implicated in various cancer types. A 4,977 bp deletion in the major arch of the mitochondrial genome is one of the most common mutations associated with a variety of human diseases and aging. METHODS: We conducted a comprehensive study on clinical features and mtDNA of 104 colorectal cancer patients in the Wenzhou area of China. In particular, using a quantitative real time PCR method, we analyzed the 4,977 bp deletion and mtDNA content in tumor tissues and paired non-tumor areas from these patients. RESULTS: We found that the 4,977 bp deletion was more likely to be present in patients of younger age (≤65 years, p = 0.027). In patients with the 4,977 bp deletion, the deletion level decreased as the cancer stage advanced (p = 0.031). Moreover, mtDNA copy number in tumor tissues of patients with this deletion increased, both compared with that in adjacent non-tumor tissues and with in tumors of patients without the deletion. Such mtDNA content increase correlated with the levels of the 4,977 bp deletion and with cancer stage (p < 0.001). CONCLUSIONS: Our study indicates that the mtDNA 4,977 bp deletion may play a role in the early stage of colorectal cancer, and it is also implicated in alteration of mtDNA content in cancer cells. BioMed Central 2011-01-13 /pmc/articles/PMC3025938/ /pubmed/21232124 http://dx.doi.org/10.1186/1471-2350-12-8 Text en Copyright ©2011 Chen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Tao He, Jing Shen, Lijun Fang, Hezhi Nie, Hezhongrong Jin, Tao Wei, Xiaosong Xin, Yijuan Jiang, Yulin Li, Hongzhi Chen, Guorong Lu, Jianxin Bai, Yidong The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
title | The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
title_full | The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
title_fullStr | The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
title_full_unstemmed | The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
title_short | The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
title_sort | mitochondrial dna 4,977-bp deletion and its implication in copy number alteration in colorectal cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025938/ https://www.ncbi.nlm.nih.gov/pubmed/21232124 http://dx.doi.org/10.1186/1471-2350-12-8 |
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