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A rapid method to screen putative mRNA targets of any known microRNA

BACKGROUND: microRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression by binding to specific sequences on target mRNAs. However, functional identification of mRNA targets is usually difficult and time consuming. Here we report hybrid-PCR as a new and rapid approach to screen pu...

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Detalles Bibliográficos
Autores principales: Huang, Yujing, Qi, Ying, Ruan, Qiang, Ma, Yanping, He, Rong, Ji, Yaohua, Sun, Zhengrong
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025964/
https://www.ncbi.nlm.nih.gov/pubmed/21219658
http://dx.doi.org/10.1186/1743-422X-8-8