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Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein

Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181–194). Cassava was genetically modified to exp...

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Detalles Bibliográficos
Autores principales: Abhary, Mohammad, Siritunga, Dimuth, Stevens, Gene, Taylor, Nigel J., Fauquet, Claude M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3026814/
https://www.ncbi.nlm.nih.gov/pubmed/21283593
http://dx.doi.org/10.1371/journal.pone.0016256
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author Abhary, Mohammad
Siritunga, Dimuth
Stevens, Gene
Taylor, Nigel J.
Fauquet, Claude M.
author_facet Abhary, Mohammad
Siritunga, Dimuth
Stevens, Gene
Taylor, Nigel J.
Fauquet, Claude M.
author_sort Abhary, Mohammad
collection PubMed
description Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181–194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications.
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spelling pubmed-30268142011-01-31 Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein Abhary, Mohammad Siritunga, Dimuth Stevens, Gene Taylor, Nigel J. Fauquet, Claude M. PLoS One Research Article Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181–194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications. Public Library of Science 2011-01-25 /pmc/articles/PMC3026814/ /pubmed/21283593 http://dx.doi.org/10.1371/journal.pone.0016256 Text en Abhary et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Abhary, Mohammad
Siritunga, Dimuth
Stevens, Gene
Taylor, Nigel J.
Fauquet, Claude M.
Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein
title Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein
title_full Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein
title_fullStr Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein
title_full_unstemmed Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein
title_short Transgenic Biofortification of the Starchy Staple Cassava (Manihot esculenta) Generates a Novel Sink for Protein
title_sort transgenic biofortification of the starchy staple cassava (manihot esculenta) generates a novel sink for protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3026814/
https://www.ncbi.nlm.nih.gov/pubmed/21283593
http://dx.doi.org/10.1371/journal.pone.0016256
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