Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
Termination of transcription is an important component of bacterial gene expression. However, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, Rickettsia prowazekii. To assess transcriptional termination in this bacterium, transcripts...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3027695/ https://www.ncbi.nlm.nih.gov/pubmed/21298070 http://dx.doi.org/10.1371/journal.pone.0016537 |
_version_ | 1782197157518901248 |
---|---|
author | Woodard, Andrew Wood, David O. |
author_facet | Woodard, Andrew Wood, David O. |
author_sort | Woodard, Andrew |
collection | PubMed |
description | Termination of transcription is an important component of bacterial gene expression. However, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, Rickettsia prowazekii. To assess transcriptional termination in this bacterium, transcripts of convergent gene pairs, some containing predicted intrinsic terminators, were analyzed. These analyses revealed that, rather than terminating at a specific site within the intervening region between the convergent genes, most of the transcripts demonstrated either a lack of termination within this region, which generated antisense RNA, or a putative non-site-specific termination that occurred throughout the intervening sequence. Transcripts terminating at predicted intrinsic terminators, as well as at a putative Rho-dependant terminator, were also examined and found to vary based on the rickettsial host environment. These results suggest that transcriptional termination, or lack thereof, plays a role in rickettsial gene regulation. |
format | Text |
id | pubmed-3027695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30276952011-02-04 Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii Woodard, Andrew Wood, David O. PLoS One Research Article Termination of transcription is an important component of bacterial gene expression. However, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, Rickettsia prowazekii. To assess transcriptional termination in this bacterium, transcripts of convergent gene pairs, some containing predicted intrinsic terminators, were analyzed. These analyses revealed that, rather than terminating at a specific site within the intervening region between the convergent genes, most of the transcripts demonstrated either a lack of termination within this region, which generated antisense RNA, or a putative non-site-specific termination that occurred throughout the intervening sequence. Transcripts terminating at predicted intrinsic terminators, as well as at a putative Rho-dependant terminator, were also examined and found to vary based on the rickettsial host environment. These results suggest that transcriptional termination, or lack thereof, plays a role in rickettsial gene regulation. Public Library of Science 2011-01-26 /pmc/articles/PMC3027695/ /pubmed/21298070 http://dx.doi.org/10.1371/journal.pone.0016537 Text en Woodard, Wood. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Woodard, Andrew Wood, David O. Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii |
title | Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
|
title_full | Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
|
title_fullStr | Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
|
title_full_unstemmed | Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
|
title_short | Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
|
title_sort | analysis of convergent gene transcripts in the obligate intracellular bacterium rickettsia prowazekii |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3027695/ https://www.ncbi.nlm.nih.gov/pubmed/21298070 http://dx.doi.org/10.1371/journal.pone.0016537 |
work_keys_str_mv | AT woodardandrew analysisofconvergentgenetranscriptsintheobligateintracellularbacteriumrickettsiaprowazekii AT wooddavido analysisofconvergentgenetranscriptsintheobligateintracellularbacteriumrickettsiaprowazekii |