Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii

Termination of transcription is an important component of bacterial gene expression. However, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, Rickettsia prowazekii. To assess transcriptional termination in this bacterium, transcripts...

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Autores principales: Woodard, Andrew, Wood, David O.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3027695/
https://www.ncbi.nlm.nih.gov/pubmed/21298070
http://dx.doi.org/10.1371/journal.pone.0016537
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author Woodard, Andrew
Wood, David O.
author_facet Woodard, Andrew
Wood, David O.
author_sort Woodard, Andrew
collection PubMed
description Termination of transcription is an important component of bacterial gene expression. However, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, Rickettsia prowazekii. To assess transcriptional termination in this bacterium, transcripts of convergent gene pairs, some containing predicted intrinsic terminators, were analyzed. These analyses revealed that, rather than terminating at a specific site within the intervening region between the convergent genes, most of the transcripts demonstrated either a lack of termination within this region, which generated antisense RNA, or a putative non-site-specific termination that occurred throughout the intervening sequence. Transcripts terminating at predicted intrinsic terminators, as well as at a putative Rho-dependant terminator, were also examined and found to vary based on the rickettsial host environment. These results suggest that transcriptional termination, or lack thereof, plays a role in rickettsial gene regulation.
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spelling pubmed-30276952011-02-04 Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii Woodard, Andrew Wood, David O. PLoS One Research Article Termination of transcription is an important component of bacterial gene expression. However, little is known concerning this process in the obligate intracellular pathogen and model for reductive evolution, Rickettsia prowazekii. To assess transcriptional termination in this bacterium, transcripts of convergent gene pairs, some containing predicted intrinsic terminators, were analyzed. These analyses revealed that, rather than terminating at a specific site within the intervening region between the convergent genes, most of the transcripts demonstrated either a lack of termination within this region, which generated antisense RNA, or a putative non-site-specific termination that occurred throughout the intervening sequence. Transcripts terminating at predicted intrinsic terminators, as well as at a putative Rho-dependant terminator, were also examined and found to vary based on the rickettsial host environment. These results suggest that transcriptional termination, or lack thereof, plays a role in rickettsial gene regulation. Public Library of Science 2011-01-26 /pmc/articles/PMC3027695/ /pubmed/21298070 http://dx.doi.org/10.1371/journal.pone.0016537 Text en Woodard, Wood. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Woodard, Andrew
Wood, David O.
Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
title Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
title_full Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
title_fullStr Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
title_full_unstemmed Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
title_short Analysis of Convergent Gene Transcripts in the Obligate Intracellular Bacterium Rickettsia prowazekii
title_sort analysis of convergent gene transcripts in the obligate intracellular bacterium rickettsia prowazekii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3027695/
https://www.ncbi.nlm.nih.gov/pubmed/21298070
http://dx.doi.org/10.1371/journal.pone.0016537
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