Improved Precursor Directed Biosynthesis in E. coli via Directed Evolution

Erythromycin and related macrolide antibiotics are widely used polyketide natural products. We have evolved an engineered biosynthetic pathway in Escherichia coli that yields erythromycin analogs from simple synthetic precursors. Multiple rounds of mutagenesis and screening led to the identification...

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Detalles Bibliográficos
Autores principales: Lee, Ho Young, Harvey, Colin J.B., Cane, David E., Khosla, Chaitan
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030684/
https://www.ncbi.nlm.nih.gov/pubmed/21081955
http://dx.doi.org/10.1038/ja.2010.129

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030684/
https://www.ncbi.nlm.nih.gov/pubmed/21081955
http://dx.doi.org/10.1038/ja.2010.129

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