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Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typi...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228/ https://www.ncbi.nlm.nih.gov/pubmed/21314970 http://dx.doi.org/10.1186/1297-9716-42-8 |