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Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection

Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typi...

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Detalles Bibliográficos
Autores principales: Løvoll, Marie, Austbø, Lars, Jørgensen, Jorunn B, Rimstad, Espen, Frost, Petter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031228/
https://www.ncbi.nlm.nih.gov/pubmed/21314970
http://dx.doi.org/10.1186/1297-9716-42-8