Lesson from the Stoichiometry Determination of the Cohesin Complex: A Short Protease Mediated Elution Increases the Recovery from Cross-Linked Antibody-Conjugated Beads

[Image: see text] Affinity purification of proteins using antibodies coupled to beads and subsequent mass spectrometric analysis has become a standard technique for the identification of protein complexes. With the recent transfer of the isotope dilution mass spectrometry principle (IDMS) to the fie...

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Detalles Bibliográficos
Autores principales: Holzmann, Johann, Fuchs, Johannes, Pichler, Peter, Peters, Jan-Michael, Mechtler, Karl
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society 2010
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033704/
https://www.ncbi.nlm.nih.gov/pubmed/21043528
http://dx.doi.org/10.1021/pr100927x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033704/
https://www.ncbi.nlm.nih.gov/pubmed/21043528
http://dx.doi.org/10.1021/pr100927x

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