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Isolation of Bacterial Ribosomes with Monolith Chromatography

We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify riboso...

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Detalles Bibliográficos
Autores principales: Trauner, Andrej, Bennett, Mark H., Williams, Huw D.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033897/
https://www.ncbi.nlm.nih.gov/pubmed/21326610
http://dx.doi.org/10.1371/journal.pone.0016273