Isolation of Bacterial Ribosomes with Monolith Chromatography

We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify riboso...

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Detalles Bibliográficos
Autores principales: Trauner, Andrej, Bennett, Mark H., Williams, Huw D.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033897/
https://www.ncbi.nlm.nih.gov/pubmed/21326610
http://dx.doi.org/10.1371/journal.pone.0016273
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author Trauner, Andrej
Bennett, Mark H.
Williams, Huw D.
author_facet Trauner, Andrej
Bennett, Mark H.
Williams, Huw D.
author_sort Trauner, Andrej
collection PubMed
description We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology.
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spelling pubmed-30338972011-02-15 Isolation of Bacterial Ribosomes with Monolith Chromatography Trauner, Andrej Bennett, Mark H. Williams, Huw D. PLoS One Research Article We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology. Public Library of Science 2011-02-04 /pmc/articles/PMC3033897/ /pubmed/21326610 http://dx.doi.org/10.1371/journal.pone.0016273 Text en Trauner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Trauner, Andrej
Bennett, Mark H.
Williams, Huw D.
Isolation of Bacterial Ribosomes with Monolith Chromatography
title Isolation of Bacterial Ribosomes with Monolith Chromatography
title_full Isolation of Bacterial Ribosomes with Monolith Chromatography
title_fullStr Isolation of Bacterial Ribosomes with Monolith Chromatography
title_full_unstemmed Isolation of Bacterial Ribosomes with Monolith Chromatography
title_short Isolation of Bacterial Ribosomes with Monolith Chromatography
title_sort isolation of bacterial ribosomes with monolith chromatography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033897/
https://www.ncbi.nlm.nih.gov/pubmed/21326610
http://dx.doi.org/10.1371/journal.pone.0016273
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