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Isolation of Bacterial Ribosomes with Monolith Chromatography
We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify riboso...
Autores principales: | Trauner, Andrej, Bennett, Mark H., Williams, Huw D. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033897/ https://www.ncbi.nlm.nih.gov/pubmed/21326610 http://dx.doi.org/10.1371/journal.pone.0016273 |
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