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Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term
The placenta contains a large variety of metabolizing enzymes, among them UDP-glucuronosyltransferase (UGT). Several UGT2B isozymes have so far been detected in human placenta, but little is known on placental expression of UGT1A isozymes. The antiepileptic drug lamotrigine (LTG) is a UGT1A4-substra...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034912/ https://www.ncbi.nlm.nih.gov/pubmed/21302032 http://dx.doi.org/10.1007/s13318-010-0021-x |
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author | Reimers, Arne Østby, Lene Stuen, Ina Sundby, Eirik |
author_facet | Reimers, Arne Østby, Lene Stuen, Ina Sundby, Eirik |
author_sort | Reimers, Arne |
collection | PubMed |
description | The placenta contains a large variety of metabolizing enzymes, among them UDP-glucuronosyltransferase (UGT). Several UGT2B isozymes have so far been detected in human placenta, but little is known on placental expression of UGT1A isozymes. The antiepileptic drug lamotrigine (LTG) is a UGT1A4-substrate, and its serum concentration falls by over 50% during pregnancy, leading to impaired seizure control. The placenta may be involved in this. Microsomes from term placentas of 4 LTG-users and 10 healthy control subjects were prepared. Western blot analysis detected UGT1A proteins in all placentas. The presence of UGT1A4 in placenta from LTG users was confirmed with UGT1A4 commercial standard and a specific UGT1A4 primary antibody. Since LTG is primarily metabolized by UGT1A4 and this isozyme is shown to be present in placenta at term, it may be hypothesized that the placenta is involved in the fall of LTG serum concentrations during pregnancy. |
format | Text |
id | pubmed-3034912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-30349122011-03-16 Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term Reimers, Arne Østby, Lene Stuen, Ina Sundby, Eirik Eur J Drug Metab Pharmacokinet Original Paper The placenta contains a large variety of metabolizing enzymes, among them UDP-glucuronosyltransferase (UGT). Several UGT2B isozymes have so far been detected in human placenta, but little is known on placental expression of UGT1A isozymes. The antiepileptic drug lamotrigine (LTG) is a UGT1A4-substrate, and its serum concentration falls by over 50% during pregnancy, leading to impaired seizure control. The placenta may be involved in this. Microsomes from term placentas of 4 LTG-users and 10 healthy control subjects were prepared. Western blot analysis detected UGT1A proteins in all placentas. The presence of UGT1A4 in placenta from LTG users was confirmed with UGT1A4 commercial standard and a specific UGT1A4 primary antibody. Since LTG is primarily metabolized by UGT1A4 and this isozyme is shown to be present in placenta at term, it may be hypothesized that the placenta is involved in the fall of LTG serum concentrations during pregnancy. Springer-Verlag 2010-12-30 2011 /pmc/articles/PMC3034912/ /pubmed/21302032 http://dx.doi.org/10.1007/s13318-010-0021-x Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Paper Reimers, Arne Østby, Lene Stuen, Ina Sundby, Eirik Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
title | Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
title_full | Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
title_fullStr | Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
title_full_unstemmed | Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
title_short | Expression of UDP-glucuronosyltransferase 1A4 in human placenta at term |
title_sort | expression of udp-glucuronosyltransferase 1a4 in human placenta at term |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3034912/ https://www.ncbi.nlm.nih.gov/pubmed/21302032 http://dx.doi.org/10.1007/s13318-010-0021-x |
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