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Efficient preparation of internally modified single-molecule constructs using nicking enzymes
Investigations of enzymes involved in DNA metabolism have strongly benefited from the establishment of single molecule techniques. These experiments frequently require elaborate DNA substrates, which carry chemical labels or nucleic acid tertiary structures. Preparing such constructs often represent...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035433/ https://www.ncbi.nlm.nih.gov/pubmed/21071409 http://dx.doi.org/10.1093/nar/gkq1004 |