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Efficient preparation of internally modified single-molecule constructs using nicking enzymes

Investigations of enzymes involved in DNA metabolism have strongly benefited from the establishment of single molecule techniques. These experiments frequently require elaborate DNA substrates, which carry chemical labels or nucleic acid tertiary structures. Preparing such constructs often represent...

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Detalles Bibliográficos
Autores principales: Luzzietti, Nicholas, Brutzer, Hergen, Klaue, Daniel, Schwarz, Friedrich W., Staroske, Wolfgang, Clausing, Sylvia, Seidel, Ralf
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035433/
https://www.ncbi.nlm.nih.gov/pubmed/21071409
http://dx.doi.org/10.1093/nar/gkq1004