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Design, construction and characterization of a set of insulated bacterial promoters

We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design...

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Detalles Bibliográficos
Autores principales: Davis, Joseph H., Rubin, Adam J., Sauer, Robert T.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035448/
https://www.ncbi.nlm.nih.gov/pubmed/20843779
http://dx.doi.org/10.1093/nar/gkq810