Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template

Gene targeting by homologous recombination (HR) can be induced by double-strand breaks (DSBs), however these breaks can be toxic and potentially mutagenic. We investigated the I-AniI homing endonuclease engineered to produce only nicks, and found that nicks induce HR with both plasmid and adeno-asso...

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Detalles Bibliográficos
Autores principales: Metzger, Michael J., McConnell-Smith, Audrey, Stoddard, Barry L., Miller, A. Dusty
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035452/
https://www.ncbi.nlm.nih.gov/pubmed/20876694
http://dx.doi.org/10.1093/nar/gkq826

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035452/
https://www.ncbi.nlm.nih.gov/pubmed/20876694
http://dx.doi.org/10.1093/nar/gkq826

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