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Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template
Gene targeting by homologous recombination (HR) can be induced by double-strand breaks (DSBs), however these breaks can be toxic and potentially mutagenic. We investigated the I-AniI homing endonuclease engineered to produce only nicks, and found that nicks induce HR with both plasmid and adeno-asso...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3035452/ https://www.ncbi.nlm.nih.gov/pubmed/20876694 http://dx.doi.org/10.1093/nar/gkq826 |