Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription

BACKGROUND: Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer. RESULTS: Using an integrative approach that combine...

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Detalles Bibliográficos
Autores principales: Hulf, Toby, Sibbritt, Tennille, Wiklund, Erik D, Bert, Saul, Strbenac, Dario, Statham, Aaron L, Robinson, Mark D, Clark, Susan J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3037319/
https://www.ncbi.nlm.nih.gov/pubmed/21255435
http://dx.doi.org/10.1186/1471-2164-12-54
Descripción
Sumario:BACKGROUND: Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer. RESULTS: Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells. CONCLUSIONS: We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression.

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