Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels

The use of high stringency selection systems commonly results in a strongly diminished number of stably transfected mammalian cell lines. Here we placed twelve different promoters upstream of an adjacent primary promoter and tested whether this might result in an increased number of colonies; this i...

Descripción completa

Detalles Bibliográficos
Autores principales: Hoeksema, F., Hamer, K., Siep, M., Verhees, J. A., Otte, A. P.
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039411/
https://www.ncbi.nlm.nih.gov/pubmed/21350661
http://dx.doi.org/10.4061/2011/492875

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039411/
https://www.ncbi.nlm.nih.gov/pubmed/21350661
http://dx.doi.org/10.4061/2011/492875

Ejemplares similares