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The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons
The neurofilament light subunit (NF-L) binds to myosin Va (Myo Va) in neurons but the sites of interaction and functional significance are not clear. We show by deletion analysis that motor domain of Myo Va binds to the NF-L rod domain that forms the NF backbone. Loss of NF-L and Myo Va binding from...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040190/ https://www.ncbi.nlm.nih.gov/pubmed/21359212 http://dx.doi.org/10.1371/journal.pone.0017087 |
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author | Rao, Mala V. Mohan, Panaiyur S. Kumar, Asok Yuan, Aidong Montagna, Lee Campbell, Jabbar Veeranna, Espreafico, Enilza M. Julien, Jean P. Nixon, Ralph A. |
author_facet | Rao, Mala V. Mohan, Panaiyur S. Kumar, Asok Yuan, Aidong Montagna, Lee Campbell, Jabbar Veeranna, Espreafico, Enilza M. Julien, Jean P. Nixon, Ralph A. |
author_sort | Rao, Mala V. |
collection | PubMed |
description | The neurofilament light subunit (NF-L) binds to myosin Va (Myo Va) in neurons but the sites of interaction and functional significance are not clear. We show by deletion analysis that motor domain of Myo Va binds to the NF-L rod domain that forms the NF backbone. Loss of NF-L and Myo Va binding from axons significantly reduces the axonal content of ER, and redistributes ER to the periphery of axon. Our data are consistent with a novel function for NFs as a scaffold in axons for maintaining the content and proper distribution of vesicular organelles, mediated in part by Myo Va. Based on observations that the Myo Va motor domain binds to intermediate filament (IF) proteins of several classes, Myo Va interactions with IFs may serve similar roles in organizing organelle topography in different cell types. |
format | Text |
id | pubmed-3040190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30401902011-02-25 The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons Rao, Mala V. Mohan, Panaiyur S. Kumar, Asok Yuan, Aidong Montagna, Lee Campbell, Jabbar Veeranna, Espreafico, Enilza M. Julien, Jean P. Nixon, Ralph A. PLoS One Research Article The neurofilament light subunit (NF-L) binds to myosin Va (Myo Va) in neurons but the sites of interaction and functional significance are not clear. We show by deletion analysis that motor domain of Myo Va binds to the NF-L rod domain that forms the NF backbone. Loss of NF-L and Myo Va binding from axons significantly reduces the axonal content of ER, and redistributes ER to the periphery of axon. Our data are consistent with a novel function for NFs as a scaffold in axons for maintaining the content and proper distribution of vesicular organelles, mediated in part by Myo Va. Based on observations that the Myo Va motor domain binds to intermediate filament (IF) proteins of several classes, Myo Va interactions with IFs may serve similar roles in organizing organelle topography in different cell types. Public Library of Science 2011-02-16 /pmc/articles/PMC3040190/ /pubmed/21359212 http://dx.doi.org/10.1371/journal.pone.0017087 Text en Rao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rao, Mala V. Mohan, Panaiyur S. Kumar, Asok Yuan, Aidong Montagna, Lee Campbell, Jabbar Veeranna, Espreafico, Enilza M. Julien, Jean P. Nixon, Ralph A. The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons |
title | The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons |
title_full | The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons |
title_fullStr | The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons |
title_full_unstemmed | The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons |
title_short | The Myosin Va Head Domain Binds to the Neurofilament-L Rod and Modulates Endoplasmic Reticulum (ER) Content and Distribution within Axons |
title_sort | myosin va head domain binds to the neurofilament-l rod and modulates endoplasmic reticulum (er) content and distribution within axons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040190/ https://www.ncbi.nlm.nih.gov/pubmed/21359212 http://dx.doi.org/10.1371/journal.pone.0017087 |
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