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High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins

We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography–mass spectrometry (LC–MS) with methanol as the organic mobile phase. Membrane proteins and detergents are separated chromatographically during the isocrati...

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Detalles Bibliográficos
Autores principales: Berridge, Georgina, Chalk, Rod, D’Avanzo, Nazzareno, Dong, Liang, Doyle, Declan, Kim, Jung-In, Xia, Xiaobing, Burgess-Brown, Nicola, deRiso, Antonio, Carpenter, Elisabeth Paula, Gileadi, Opher
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041925/
https://www.ncbi.nlm.nih.gov/pubmed/21093405
http://dx.doi.org/10.1016/j.ab.2010.11.008