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High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins
We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography–mass spectrometry (LC–MS) with methanol as the organic mobile phase. Membrane proteins and detergents are separated chromatographically during the isocrati...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3041925/ https://www.ncbi.nlm.nih.gov/pubmed/21093405 http://dx.doi.org/10.1016/j.ab.2010.11.008 |