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Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells

c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering assoc...

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Autores principales: Sancier, Florence, Dumont, Aurélie, Sirvent, Audrey, Paquay de Plater, Ludmilla, Edmonds, Thomas, David, Géraldine, Jan, Michel, de Montrion, Catherine, Cogé, Francis, Léonce, Stéphane, Burbridge, Michael, Bruno, Alain, Boutin, Jean A., Lockhart, Brian, Roche, Serge, Cruzalegui, Francisco
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044743/
https://www.ncbi.nlm.nih.gov/pubmed/21390316
http://dx.doi.org/10.1371/journal.pone.0017237
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author Sancier, Florence
Dumont, Aurélie
Sirvent, Audrey
Paquay de Plater, Ludmilla
Edmonds, Thomas
David, Géraldine
Jan, Michel
de Montrion, Catherine
Cogé, Francis
Léonce, Stéphane
Burbridge, Michael
Bruno, Alain
Boutin, Jean A.
Lockhart, Brian
Roche, Serge
Cruzalegui, Francisco
author_facet Sancier, Florence
Dumont, Aurélie
Sirvent, Audrey
Paquay de Plater, Ludmilla
Edmonds, Thomas
David, Géraldine
Jan, Michel
de Montrion, Catherine
Cogé, Francis
Léonce, Stéphane
Burbridge, Michael
Bruno, Alain
Boutin, Jean A.
Lockhart, Brian
Roche, Serge
Cruzalegui, Francisco
author_sort Sancier, Florence
collection PubMed
description c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering associated with a localisation of β-catenin at cell membranes and a reduction of expression of β-catenin target genes. c-Yes silencing induced an increase in apoptosis, inhibition of growth in soft-agar and in mouse xenografts, inhibition of cell migration and loss of the capacity to generate liver metastases in mice. Re-introduction of c-Yes, but not c -Src, restores transforming properties of c-Yes depleted cells. Moreover, we found that c-Yes kinase activity is required for its role in β-catenin localisation and growth in soft agar, whereas kinase activity is dispensable for its role in cell migration. We conclude that c-Yes regulates specific oncogenic signalling pathways important for colon cancer progression that is not shared with c-Src.
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spelling pubmed-30447432011-03-09 Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells Sancier, Florence Dumont, Aurélie Sirvent, Audrey Paquay de Plater, Ludmilla Edmonds, Thomas David, Géraldine Jan, Michel de Montrion, Catherine Cogé, Francis Léonce, Stéphane Burbridge, Michael Bruno, Alain Boutin, Jean A. Lockhart, Brian Roche, Serge Cruzalegui, Francisco PLoS One Research Article c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering associated with a localisation of β-catenin at cell membranes and a reduction of expression of β-catenin target genes. c-Yes silencing induced an increase in apoptosis, inhibition of growth in soft-agar and in mouse xenografts, inhibition of cell migration and loss of the capacity to generate liver metastases in mice. Re-introduction of c-Yes, but not c -Src, restores transforming properties of c-Yes depleted cells. Moreover, we found that c-Yes kinase activity is required for its role in β-catenin localisation and growth in soft agar, whereas kinase activity is dispensable for its role in cell migration. We conclude that c-Yes regulates specific oncogenic signalling pathways important for colon cancer progression that is not shared with c-Src. Public Library of Science 2011-02-24 /pmc/articles/PMC3044743/ /pubmed/21390316 http://dx.doi.org/10.1371/journal.pone.0017237 Text en Sancier et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sancier, Florence
Dumont, Aurélie
Sirvent, Audrey
Paquay de Plater, Ludmilla
Edmonds, Thomas
David, Géraldine
Jan, Michel
de Montrion, Catherine
Cogé, Francis
Léonce, Stéphane
Burbridge, Michael
Bruno, Alain
Boutin, Jean A.
Lockhart, Brian
Roche, Serge
Cruzalegui, Francisco
Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells
title Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells
title_full Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells
title_fullStr Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells
title_full_unstemmed Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells
title_short Specific Oncogenic Activity of the Src-Family Tyrosine Kinase c-Yes in Colon Carcinoma Cells
title_sort specific oncogenic activity of the src-family tyrosine kinase c-yes in colon carcinoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044743/
https://www.ncbi.nlm.nih.gov/pubmed/21390316
http://dx.doi.org/10.1371/journal.pone.0017237
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