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Digital MDA for enumeration of total nucleic acid contamination

Multiple displacement amplification (MDA) is an isothermal, sequence-independent method for the amplification of high molecular weight DNA that is driven by ϕ29 DNA polymerase (DNAP). Here we report digital MDA (dMDA), an ultrasensitive method for quantifying nucleic acid fragments of unknown sequen...

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Detalles Bibliográficos
Autores principales: Blainey, Paul C., Quake, Stephen R.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045575/
https://www.ncbi.nlm.nih.gov/pubmed/21071419
http://dx.doi.org/10.1093/nar/gkq1074