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Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

BACKGROUND: Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR...

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Detalles Bibliográficos
Autores principales: Chen, Gang, Kronenberger, Peter, Teugels, Erik, De Grève, Jacques
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047432/
https://www.ncbi.nlm.nih.gov/pubmed/21369532
http://dx.doi.org/10.1186/1480-9222-13-1