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A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification
BACKGROUND: Linear regression of efficiency (LRE) introduced a new paradigm for real-time qPCR that enables large-scale absolute quantification by eliminating the need for standard curves. Developed through the application of sigmoidal mathematics to SYBR Green I-based assays, target quantity is der...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047581/ https://www.ncbi.nlm.nih.gov/pubmed/21407812 http://dx.doi.org/10.1371/journal.pone.0017636 |