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A Java Program for LRE-Based Real-Time qPCR that Enables Large-Scale Absolute Quantification

BACKGROUND: Linear regression of efficiency (LRE) introduced a new paradigm for real-time qPCR that enables large-scale absolute quantification by eliminating the need for standard curves. Developed through the application of sigmoidal mathematics to SYBR Green I-based assays, target quantity is der...

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Detalles Bibliográficos
Autor principal: Rutledge, Robert G.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047581/
https://www.ncbi.nlm.nih.gov/pubmed/21407812
http://dx.doi.org/10.1371/journal.pone.0017636