Construction of a dengue virus type 4 reporter replicon and analysis of temperature-sensitive mutations in non-structural proteins 3 and 5

Replicon systems have been useful to study mechanisms of translation and replication of flavivirus RNAs. In this study, we constructed a dengue virus 4 replicon encoding a Renilla luciferase (R(luc)) reporter, and six single-residue substitution mutants were generated: L128F and S158P in the non-str...

Descripción completa

Detalles Bibliográficos
Autores principales: Alcaraz-Estrada, Sofia L., Manzano, Mark Irvin M., Del Angel, Rosa M., Levis, Robin, Padmanabhan, R.
Formato: Texto
Lenguaje:English
Publicado: Society for General Microbiology 2010
Materias:
Animal
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052559/
https://www.ncbi.nlm.nih.gov/pubmed/20631089
http://dx.doi.org/10.1099/vir.0.024083-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052559/
https://www.ncbi.nlm.nih.gov/pubmed/20631089
http://dx.doi.org/10.1099/vir.0.024083-0

Ejemplares similares