Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos

BACKGROUND: Although the transcriptome of minute quantities of cells can be profiled using nucleic acid amplification techniques, it remains difficult to distinguish between active and stored messenger RNA. Transcript storage occurs at specific stages of gametogenesis and is particularly important i...

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Detalles Bibliográficos
Autores principales: Scantland, Sara, Grenon, Jean-Philippe, Desrochers, Marie-Hélène, Sirard, Marc-André, Khandjian, Edward W, Robert, Claude
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055227/
https://www.ncbi.nlm.nih.gov/pubmed/21324132
http://dx.doi.org/10.1186/1471-213X-11-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055227/
https://www.ncbi.nlm.nih.gov/pubmed/21324132
http://dx.doi.org/10.1186/1471-213X-11-8

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