Cargando…
Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos
BACKGROUND: Although the transcriptome of minute quantities of cells can be profiled using nucleic acid amplification techniques, it remains difficult to distinguish between active and stored messenger RNA. Transcript storage occurs at specific stages of gametogenesis and is particularly important i...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055227/ https://www.ncbi.nlm.nih.gov/pubmed/21324132 http://dx.doi.org/10.1186/1471-213X-11-8 |