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Exploration of twin‐arginine translocation for expression and purification of correctly folded proteins in Escherichia coli

Historically, the general secretory (Sec) pathway of Gram‐negative bacteria has served as the primary route by which heterologous proteins are delivered to the periplasm in numerous expression and engineering applications. Here we have systematically examined the twin‐arginine translocation (Tat) pa...

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Detalles Bibliográficos
Autores principales: Fisher, Adam C., Kim, Jae‐Young, Perez‐Rodriguez, Ritsdeliz, Tullman‐Ercek, Danielle, Fish, Wallace R., Henderson, Lee A., DeLisa, Matthew P.
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057487/
https://www.ncbi.nlm.nih.gov/pubmed/21261860
http://dx.doi.org/10.1111/j.1751-7915.2008.00041.x